ارزیابی چند عصاره‌گیر شیمیایی برای تعیین مس قابل استفاده لوبیا (Phaseolus vulgaris L.) در خاک‌های تیمار شده و تیمار نشده با لجن فاضلاب شهری

Determination of copper (Cu) availability in soils is important in preferment quality and quantity of agricultural crops. To this aim, suitable extractant must be introduced. The objective of this greenhouse experiment was to evaluate several extractants to predict available Cu of cultivated bean, in 10 calcareous soils, untreated and treated with municipal sewage sludge. After one month of incubation of soils, available Cu was determined using 7 chemical procedures (DTPA-TEA, AB-DTPA, Mehlich 1, Mehlich 2, Mehlich 3, 0.1 N HCl and 0.01 M CaCl2). The results showed that Mehlich 3 and 0.01 M CaCl2 in treated and untreated soils extracted the highest and the lowest amount of Cu, respectively. Among the extractants used for untreated soils, significant correlation was found between extracted Cu using DTPA-TEA with concentration and uptake of Cu in shoots. In addition, in sewage sludge-amended soils, correlation between extracted Cu using DTPA-TEA, AB-DTPA and Mehlich 3 and concentration, dry matter and uptake of Cu was significant. In general, the results of this research showed that DTPA-TEA could be used to predict available Cu in untreated and sewage sludge-treated calcareous soils under bean cultivation.